Immunoblot analysis using antigen from Taenia crassiceps cysticerci in the diagnosis of swine cysticercosis
نویسندگان
چکیده
منابع مشابه
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci.
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia so...
متن کاملSerodiagnosis of human cysticercosis by using antigens from vesicular fluid of Taenia crassiceps cysticerci.
Neurocysticercosis (NC), caused by the presence of Taenia solium metacestodes in tissues, is a severe parasitic infection of the central nervous system with universal distribution. To determine the efficiency of enzyme-linked immunosorbent assay (ELISA) and immunoblot with antigens of T. crassiceps vesicular fluid (Tcra) compared to standard techniques (indirect immunofluorescence test [IFT] an...
متن کاملMitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)
Mitochondrial thioredoxin-glutathione reductase was purified from larval Taenia crassiceps (cysticerci). The preparation showed NADPH-dependent reductase activity with either thioredoxin or GSSG, and was able to perform thiol/disulfide exchange reactions. At 25 degrees C specific activities were 437 +/- 27 mU mg(-1) and 840 +/- 49 mU mg(-1) with thioredoxin and GSSG, respectively. Apparent K(m)...
متن کاملExperimental encephalitis caused by Taenia crassiceps cysticerci in mice.
OBJECTIVES To present the experimental model of neurocysticercosis (NCC) caused by Taenia crassiceps cysticerci, to describe the inflammatory process, susceptibility, or resistance of BALB/c and C57BL/6 mice to this infection, and to describe the host-parasite relationship. METHODS The animals were intracranially inoculated with initial stage T. crassiceps cysticerci. They were euthanized at ...
متن کاملSerological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci.
A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expres...
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ژورنال
عنوان ژورنال: Boletín chileno de parasitología
سال: 2001
ISSN: 0365-9402
DOI: 10.4067/s0365-94022001000100010